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Scheduler

The Scheduler is an option when choosing a Time Scan. The Scheduler will setup and run a sequential series of user defined Scans. The Scan may be paused at anytime during the experiment to adjust Capture Settings, including exposure and gain. To setup a Scheduled scan go to the Sequence pane and select Time Scan from the Select Scan Type drop-menu and check the Use Scheduler option. Once the scan has been setup use the Save Scan icon to save scan settings for repeated use. Use the Open icon to load a saved scan. The example below shows scans being setup and a Scan in progress.

Schedule

Each scan is defined by Name, Type and Duration which are setup by the user. Additional Scans may be added by right-clicking on Scan name and selecting Add SubScan. You may also Copy and Paste, or Remove unwanted scans in the same manor. Use the Loop controls to repeat the entire sequence of scans. Click Text View in order view the schedule at as text file for easy viewing.

Name

By default the scans are numbered sequentially, enter a custom name by double clicking on scan name and entering a new name. Left mouse click outside of the name box to save the name.

Type

Select the scan type using the Type drop menu. To access the scan controls, double-click the right-facing arrow on the left side of the panel. The dialog that opens provides the necessary controls for the type of scan selected.

• Time: Time-lapse for a set duration
• TTL In: Waits for TTL Pulse from a selected pin
• TTL Out: Outputs a TTL Pulse to selected pin
• Z Abs: Performs an Absolute Z Scan with defined start and end points
• Z Rel: Performs a Relative Z Scan, were the start and end points are dependent upon current Z position
• WL: Performs a Wavelength Scan with defined start and end wavelengths
• AE: Performs an Auto-exposure based on selected parameters
• AFocus: Performs an Autofocus based on selected parameters
• Delay: Initiates a Time Delay based on predefined duration
• TTL On: Outputs a constant TTL Pulse to a selected pin until it is turned off using TTL Off
• TTL Off: Turns off a TTL On scan
• Camera: Control various camera parameters such as exposure time and speed
• Filter: Change filter positions
• RAM to DISK: Saves images stored in memory to disk

Note: Simultaneously send TTL pulses while acquiring images using the asynchronous mode. Simply enter pulse count, duration, etc. then select Asynchronous in the TTL Out dialog. Next setup the time scan and when you click start both scans will run simultaneously.

Duration

The duration indicates how many actions will occur during a scan. For example, in a time-lapse scan, the duration indicates how many images will be acquired or how long the scan will acquire for. For TTL Out, it is the number of out pulses and the number of images to acquire for Z scans.

Progress

Displays the live progress of the scan as a percent complete.

Status

Indicates the whether the scan has started, is in progress or is complete.

Progress

• Event Markers - can be added to the data document file during acquisition as notable events occur in the experiment. Both the numbered buttons above and keyboard numbers may be used.
• Time Elapsed - The time from the start of the first scan.
• Delay Remaining - a reference to delay time. The right arrow button may be clicked to exit the delay.
• Frame Count - Total number of frames captured.
• FPS - Frames per second captured.

Scan Settings

The Scheduler Scan Settings panel is a condensed set of controls to select where and how the data will be saved.

Storage and Display

Use AutoSave to define where and how to store acquired data.

• CXD: Image data is organized and saved as a data document
• TIFF: Captured data is saved as individual image files
• MPTIFF: Captured data is saved as multipage tiffs

Determines how the data is structured when written to a data document.

• To a Single File: Store captured image data in successive fields.
• One File Per Loop: Store captured image data for each loop in a series of data documents called a Summary DocumentA Summary document (.cxs) is a collection of data documents (.cxd)..

Determines if a live view is displayed in the image display or if acquired images may be reviewed during the acquisition.

• Update Image Display: During a sequence captured images are displayed in the image display
• Review Images: During a sequence captured images may be reviewed as newly captured images are added.