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Wavelength Scan

The Wavelength Scan provides the ability to create a spectral scan through a range of wavelengths. To setup a Wavelength scan go to the Sequence pane and select Wavelength Scan from the Select Scan Type drop-menu. Once the scan has been setup use the Save Scan icon to save scan settings for repeated use. Use the Open icon to load a saved scan.

Progress

• Event Markers - Can be added to the data document file during acquisition as notable events occur in the experiment. Both the numbered buttons above and keyboard numbers may be used.
• Time Elapsed - The time from the start of the first scan.
• Delay Remaining - A reference to delay time. The right arrow button may be clicked to exit the delay.
• Frame Count - Total number of frames captured.
• FPS - Frames per second captured

Scan Settings

The Scan Settings panel is easy-to-use, simply set the speed, define the capture interval, enter the number of passes to capture and where to save the data.

Storage and Display

Use AutoSave to define where and how to store acquired data.

• CXD: Image data is organized and saved as a data document
• TIFF: Captured data is saved as individual image files
• MPTIFF: Captured data is saved as multipage tiffs

Determines how the data is structured when written to a data document.

• To a Single File: Store captured image data in successive fields.
• One File Per Pass: Store captured image data for each pass in a series of data documents called a Summary DocumentA Summary document (.cxs) is a collection of data documents (.cxd)..
• One File Per Position: Store captured image data on a positional basis. A Summary document (.cxs) is created with a Data Document (.cxd) for each position.

Determines if a live view is displayed in the image display or if acquired images may be reviewed during the acquisition.

• Update Image Display: During a sequence captured images are displayed in the image display
• Review Images: During a sequence captured images may be reviewed as newly captured images are added.

Speed

Select the maximum speed or a type of delay.

• Enable Maximum: Capture to memory at maximum speed. During maximum speed, items which slow down acquisition will be ignored (i.e., exposure protection and Z auto-focus).

• Field Delay: A pause between each frame of the scan. Enter a numerical value followed by units. (e.g., 5 seconds = 5s)

• Pass Delay: A pause between subsequent passes in a multi-pass scan. Enter a numerical value followed by units. (e.g., 2 milliseconds = 2m)
• Hours = h
• Minutes = t
• Seconds = s
• Milliseconds = m
• Microseconds = u

• Disk: Stream data directly to disk
• Memory: High speed acquisition stream to memory
• RAM: Sets the limits for the amount of RAM available for streaming

Control

Defines a specific Pass to stop the current scan.

• Continuous: The scan runs according settings without a preset endpoint. The scan will be stopped when resources run out or can be interrupted using the Finish button.
• Pass Count: Defines a specific number of passes to complete the current scan.

Wavelength Scan Setup

The Wavelength Scan Setup provides the basic controls for setting up a Wavelength scan.

Wavelength Slider

The Wavelength Slider allows the wavelength to be changed by clicking and dragging the slider handle. The movement of the slider is relative, not absolute, so dragging the slider further up or down the scale will produce a faster movement in that direction.

Top Wavelength Limit

The Top Limit can be set to prevent the scan from being incremented past a certain wavelength. Checking the Top limit will assign the current wavelength to the limit, or the wavelength can be numerically entered. Clicking the Top button will move to the wavelength in the top position.

Current Wavelength

The Current wavelength is read from the controller and displays the current wavelength. This number can also be input numerically to instruct the controller to move to a specific wavelength.

Bottom Wavelength Limit

The Bottom Limit can be set to prevent the scan from being incremented past a certain wavelength. Checking the Bottom limit will assign the current wavelength to the limit, or the wavelength can be numerically entered. Clicking the Bottom button will move to the wavelength in the bottom position.

Increment

The number of steps to move between captures.

Count

The number of steps based on the size of increment in the Wavelength scan.